KMID : 0811720140180040307
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Korean Journal of Physiology & Pharmacology 2014 Volume.18 No. 4 p.307 ~ p.311
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Scopoletin from Cirsium setidens Increases Melanin Synthesis via CREB Phosphorylation in B16F10 Cells
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Ahn Mi-Ja
Hur Sun-Jung Kim Eun-Hyun Lee Seung-Hoon Shin Jun-Seob Kim Myo-Kyoung Uchizono James A. Whang Wan Kyunn Kim Dong-Seok
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Abstract
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In this study, we isolated scopoletin from Cirsium setidens Nakai (Compositae) and tested its effects on melanogenesis. Scopoletin was not toxic to cells at concentrations less than 50 ¥ìM and increased melanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulated the total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however, scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator of tyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production of microphthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP response element-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results, preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.
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KEYWORD
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Cirsium setidens, CREB, MITF, Scopoletin, Tyrosinase
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